We further use in silico methods to investigate the linear B-cell epitopes, Cytotoxic T Lymphocytes (CTL) epitopes, Helper T Lymphocytes (HTL) epitopes in the 26 subunit candidates and identify the best 11 of them to construct a multi-epitope vaccine for SARS-CoV-2 virus. By combining the in silico immunoinformatics and deep neural network strategies, the DeepVacPred computational framework directly predicts 26 potential vaccine subunits from the available SARS-CoV-2 spike protein sequence. In this study, we propose an in silico deep learning approach for prediction and design of a multi-epitope vaccine (DeepVacPred). Without an existing effective medical therapy, vaccines are urgently needed to avoid the spread of this disease. Sticky ends from different BstEII sites may not be compatible.The rampant spread of COVID-19, an infectious disease caused by SARS-CoV-2, all over the world has led to over millions of deaths, and devastated the social, financial and political entities around the world. Sticky ends from different BstAPI sites may not be compatible.īclI is typically used at 50-55☌, but is 50% active at 37☌. G C A N N N N N T G C C G T N N N N N A C G Prolonged incubation with NdeI may lead to removal of additional nucleotides.Įfficient cleavage requires at least two copies of the SgrAI recognition sequence. Sticky ends from different Tth111I sites may not be compatible.Īfter cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility. SmaI can be used at 37☌ for brief incubations.Ĭleavage may be enhanced when more than one copy of the XmaI recognition sequence is present.Ĭ T G A A G ( N ) 14 N N G A C T T C ( N ) 14Ĭleavage may be enhanced when more than one copy of the AcuI recognition sequence is present. Sticky ends from different DraIII sites may not be compatible. Sticky ends from different BlpI sites may not be compatible. PaeR7I does not recognize the sequence CTCTCGAG.
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